RNA

Part:BBa_K4061046:Design

Designed by: Diya Agrawal   Group: iGEM21_HKUST   (2021-10-15)


Antisense RNA to constitutive mRFP1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 57
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 49
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The antisense mRNA spans 22nt of the protein CDS, the BBa_B0034 RBS and the -10 promoter region. It also contains an Hfq-binding domain on the 3' end. The promoter region is not necessary for an antisense mRNA to function, however, we included this segment to ensure efficient suppression in case we misjudge the Trascription Start Site.


Source

Biobricks and self-design

References